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ccl 235 calu1 cell line atcc  (ATCC)


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    Structured Review

    ATCC ccl 235 calu1 cell line atcc
    Ccl 235 Calu1 Cell Line Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl+235+calu1+cell+line+atcc/SW837/pm29373830-216-210-214
    Average 95 stars, based on 258 article reviews
    ccl 235 calu1 cell line atcc - by Bioz Stars, 2026-09
    95/100 stars

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    Article Title: Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LU65 cell line Japanese Collection of Research Bioresources Cell Bank (JCRB) Cat# JCRB0079 LU99A cell line Japanese Collection of Research Bioresources Cell Bank (JCRB) Cat# JCRB0044 HCC44 cell line DSMZ Cat# ACC 534 HCC1171 cell line Korean Cell Line Bank (JCLB) Cat# 71171 Experimental Models: Organisms/Strains Athymic female nude mice (NCr) nu/nu, 8-weeks old Simonsen Labs http://www.simlab.com/products/ nudes.html Male BALB/cAnNCrSim mice, 6- to 8-weeks old Simonsen Labs http://simlab.com/products.html CD-1 mice, 6- to 8-weeks old Charles Rivers https://www.criver.com/products-services/ basic-research/find-a-model/cd-1-mouse?loc=US Recombinant DNA Truncated a.a.1-169 KRAS G12C;C51S; C80L;C118S for crystallography cloned into pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Truncated a.a.1-169 KRAS G12C in pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Truncated a.a.1-169 KRAS in pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Catalytic domain (594-1049) of SOS1 in ProEX HTb Kuriyan Lab Margarit et al., 2003 Full-length N-terminal FLAG tagged KRAS G12C in pLenti6.3/TO This paper N/A Full-length N-terminal FLAG tagged KRAS G12V in pLenti6.3/TO This paper N/A MISSION pLKO.1 shKRAS#1 Sigma TRCN0000033262 MISSION pLKO.1 shKRAS#2 Sigma TRCN0000033260 MISSION pLKO.1 luciferase shRNA control Sigma Cat# SHC007 Software and Algorithms KinTek Explorer Chemical Kinetics Software KinTek https://kintekcorp.com/software/ CCP4 software for crystallography Winn et al., 2011 http://www.ccp4.ac.uk/ QIAGEN Biomedical Genomics Workbench v4.1.1 QIAGEN https://www.qiagenbioinformatics.com/ products/biomedical-genomics-workbench/ FlowJo v10.1 Flowjo https://www.flowjo.com/solutions/flowjo MassHunter Software Agilent https://www.agilent.com/en-us/products/ software-informatics/masshunter-suite/ masshunter/masshunter-software Progenesis LC-MS software for proteomics V3.0 Waters http://www.nonlinear.com/progenesis/ Proteome Discoverer software v1.4 ThermoFisher Scientific Cat# IQLAAEGABSFAKJMAUH Skyline Targeted Mass Spec Environment v3.6 MacLean et al., 2010 https://skyline.ms GSEA (Java, v3.0) Subramanian et al., 2005 http://software.broadinstitute.org/gsea/index.jsp Project DRIVE data portal McDonald et al., 2017 https://oncologynibr.shinyapps.io/drive/ MSigDB v6.0 Subramanian et al., 2005 http://software.broadinstitute.org/gsea/index.jsp Prism v7.03 GraphPad Software N/A e3 Cell 172, 578–589.e1–e9, January 25, 2018

    Article Title: Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1-(4-(6-chloro-8-fluoro-7-(2-fluorophenyl) quinazolin-4-yl)piperazin-1-yl)prop-2-en-1one (ARS-1170) This paper N/A 1-(4-(6-chloro-8-fluoro-7-(2-fluoro-6hydroxyphenyl)quinazolin-4-yl)piperazin-1-yl) prop-2-en-1-one (ARS-1323, a racemic mixture of ARS-1620/R-atropisomer) This paper N/A Labrasol (as oral vehicle) Gattefossé SAS, (Saint-Priest,France) Cat# 3074BFE, lot# 153313 Trametinib LC labs Cat# T-1823 Mant-GDP Sigma Cat# 69244 GDP Sigma Cat# G7127 GTP Sigma Cat# G8877 Guanosine 50-[b,g-imido]triphosphate trisodium salt hydrate (GppNp) Sigma Cat# G0635 Custom heavy isotopically labeled KRASG12C 1-169 his-tagged protein (Lys-13C6, 15N2 and Arg-13C6, 15N4) PROMISE Advanced Proteomics, Grenoble, France N/A Custom Isotopically labeled KRASG12C HeavyPeptide AQUA ThermoFisher Scientific N/A Iodoacetamide desthiobiotin This paper N/A Pierce High Capacity Streptavidin agarose ThermoFisher Scientific Cat# 20361 Critical Commercial Assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7573 Active Ras Detection Kit Cell Signaling Technology Cat# 8821 Deposited Data Crystallography data This paper PDB: 5V9U Transcriptome RNaseq data (fastq files and processed RPKM values) This paper GEO: GSE103021 Experimental Models: Cell Lines RAS-less mouse embryonic fibroblasts (MEFs): overexpressing KRAS 4B WT, KRAS 4B G12C, KRAS 4B G12D, and KRAS 4B G12V NIH (NCI RAS initiative at the FNLCR) Laboratories of Rachel Bagni, Matthew Holderfield, and Dan Soppet) N/A 293FT cell line for virus production ThermoFisher Scientific Cat# R70007 H358 (NCI-H358) cell line ATCC Cat# CRL-5807 MIA-PaCa2 cell line ATCC Cat# CRL-1420 SW837 cell line ATCC Cat# CCL-235 CALU1 cell line ATCC Cat# HTB-54 H23 (NCI-H23) cell line ATCC Cat# CRL-5800 H2122 (NCI-H2122) cell line ATCC Cat# CRL-5985 SW1463 cell line ATCC Cat# CCL-234 H2030 (NCI-H2030) cell line ATCC Cat# CRL-5914 H1373 (NCI-H1373) cell line ATCC Cat# CRL-5866 H1792 (NCI-H1792) cell line ATCC Cat# CRL-5895 SW1573 cell line ATCC Cat# CRL-2170 A549 cell line ATCC Cat# CCL-185 H441 (NCI-H441) cell line ATCC Cat# HTB-174 HCT116 cell line ATCC Cat# CCL-247 A375 cell line ATCC Cat# CRL-1619 (Continued on next page) Cell 172, 578–589.e1–e9, January 25, 2018 e2

    Cell Viability Assay:

    Article Title: Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LU65 cell line Japanese Collection of Research Bioresources Cell Bank (JCRB) Cat# JCRB0079 LU99A cell line Japanese Collection of Research Bioresources Cell Bank (JCRB) Cat# JCRB0044 HCC44 cell line DSMZ Cat# ACC 534 HCC1171 cell line Korean Cell Line Bank (JCLB) Cat# 71171 Experimental Models: Organisms/Strains Athymic female nude mice (NCr) nu/nu, 8-weeks old Simonsen Labs http://www.simlab.com/products/ nudes.html Male BALB/cAnNCrSim mice, 6- to 8-weeks old Simonsen Labs http://simlab.com/products.html CD-1 mice, 6- to 8-weeks old Charles Rivers https://www.criver.com/products-services/ basic-research/find-a-model/cd-1-mouse?loc=US Recombinant DNA Truncated a.a.1-169 KRAS G12C;C51S; C80L;C118S for crystallography cloned into pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Truncated a.a.1-169 KRAS G12C in pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Truncated a.a.1-169 KRAS in pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Catalytic domain (594-1049) of SOS1 in ProEX HTb Kuriyan Lab Margarit et al., 2003 Full-length N-terminal FLAG tagged KRAS G12C in pLenti6.3/TO This paper N/A Full-length N-terminal FLAG tagged KRAS G12V in pLenti6.3/TO This paper N/A MISSION pLKO.1 shKRAS#1 Sigma TRCN0000033262 MISSION pLKO.1 shKRAS#2 Sigma TRCN0000033260 MISSION pLKO.1 luciferase shRNA control Sigma Cat# SHC007 Software and Algorithms KinTek Explorer Chemical Kinetics Software KinTek https://kintekcorp.com/software/ CCP4 software for crystallography Winn et al., 2011 http://www.ccp4.ac.uk/ QIAGEN Biomedical Genomics Workbench v4.1.1 QIAGEN https://www.qiagenbioinformatics.com/ products/biomedical-genomics-workbench/ FlowJo v10.1 Flowjo https://www.flowjo.com/solutions/flowjo MassHunter Software Agilent https://www.agilent.com/en-us/products/ software-informatics/masshunter-suite/ masshunter/masshunter-software Progenesis LC-MS software for proteomics V3.0 Waters http://www.nonlinear.com/progenesis/ Proteome Discoverer software v1.4 ThermoFisher Scientific Cat# IQLAAEGABSFAKJMAUH Skyline Targeted Mass Spec Environment v3.6 MacLean et al., 2010 https://skyline.ms GSEA (Java, v3.0) Subramanian et al., 2005 http://software.broadinstitute.org/gsea/index.jsp Project DRIVE data portal McDonald et al., 2017 https://oncologynibr.shinyapps.io/drive/ MSigDB v6.0 Subramanian et al., 2005 http://software.broadinstitute.org/gsea/index.jsp Prism v7.03 GraphPad Software N/A e3 Cell 172, 578–589.e1–e9, January 25, 2018

    Article Title: Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1-(4-(6-chloro-8-fluoro-7-(2-fluorophenyl) quinazolin-4-yl)piperazin-1-yl)prop-2-en-1one (ARS-1170) This paper N/A 1-(4-(6-chloro-8-fluoro-7-(2-fluoro-6hydroxyphenyl)quinazolin-4-yl)piperazin-1-yl) prop-2-en-1-one (ARS-1323, a racemic mixture of ARS-1620/R-atropisomer) This paper N/A Labrasol (as oral vehicle) Gattefossé SAS, (Saint-Priest,France) Cat# 3074BFE, lot# 153313 Trametinib LC labs Cat# T-1823 Mant-GDP Sigma Cat# 69244 GDP Sigma Cat# G7127 GTP Sigma Cat# G8877 Guanosine 50-[b,g-imido]triphosphate trisodium salt hydrate (GppNp) Sigma Cat# G0635 Custom heavy isotopically labeled KRASG12C 1-169 his-tagged protein (Lys-13C6, 15N2 and Arg-13C6, 15N4) PROMISE Advanced Proteomics, Grenoble, France N/A Custom Isotopically labeled KRASG12C HeavyPeptide AQUA ThermoFisher Scientific N/A Iodoacetamide desthiobiotin This paper N/A Pierce High Capacity Streptavidin agarose ThermoFisher Scientific Cat# 20361 Critical Commercial Assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7573 Active Ras Detection Kit Cell Signaling Technology Cat# 8821 Deposited Data Crystallography data This paper PDB: 5V9U Transcriptome RNaseq data (fastq files and processed RPKM values) This paper GEO: GSE103021 Experimental Models: Cell Lines RAS-less mouse embryonic fibroblasts (MEFs): overexpressing KRAS 4B WT, KRAS 4B G12C, KRAS 4B G12D, and KRAS 4B G12V NIH (NCI RAS initiative at the FNLCR) Laboratories of Rachel Bagni, Matthew Holderfield, and Dan Soppet) N/A 293FT cell line for virus production ThermoFisher Scientific Cat# R70007 H358 (NCI-H358) cell line ATCC Cat# CRL-5807 MIA-PaCa2 cell line ATCC Cat# CRL-1420 SW837 cell line ATCC Cat# CCL-235 CALU1 cell line ATCC Cat# HTB-54 H23 (NCI-H23) cell line ATCC Cat# CRL-5800 H2122 (NCI-H2122) cell line ATCC Cat# CRL-5985 SW1463 cell line ATCC Cat# CCL-234 H2030 (NCI-H2030) cell line ATCC Cat# CRL-5914 H1373 (NCI-H1373) cell line ATCC Cat# CRL-5866 H1792 (NCI-H1792) cell line ATCC Cat# CRL-5895 SW1573 cell line ATCC Cat# CRL-2170 A549 cell line ATCC Cat# CCL-185 H441 (NCI-H441) cell line ATCC Cat# HTB-174 HCT116 cell line ATCC Cat# CCL-247 A375 cell line ATCC Cat# CRL-1619 (Continued on next page) Cell 172, 578–589.e1–e9, January 25, 2018 e2

    Virus:

    Article Title: Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LU65 cell line Japanese Collection of Research Bioresources Cell Bank (JCRB) Cat# JCRB0079 LU99A cell line Japanese Collection of Research Bioresources Cell Bank (JCRB) Cat# JCRB0044 HCC44 cell line DSMZ Cat# ACC 534 HCC1171 cell line Korean Cell Line Bank (JCLB) Cat# 71171 Experimental Models: Organisms/Strains Athymic female nude mice (NCr) nu/nu, 8-weeks old Simonsen Labs http://www.simlab.com/products/ nudes.html Male BALB/cAnNCrSim mice, 6- to 8-weeks old Simonsen Labs http://simlab.com/products.html CD-1 mice, 6- to 8-weeks old Charles Rivers https://www.criver.com/products-services/ basic-research/find-a-model/cd-1-mouse?loc=US Recombinant DNA Truncated a.a.1-169 KRAS G12C;C51S; C80L;C118S for crystallography cloned into pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Truncated a.a.1-169 KRAS G12C in pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Truncated a.a.1-169 KRAS in pJexpress411 DNA2.0/Shokat Lab Ostrem et al., 2013 Catalytic domain (594-1049) of SOS1 in ProEX HTb Kuriyan Lab Margarit et al., 2003 Full-length N-terminal FLAG tagged KRAS G12C in pLenti6.3/TO This paper N/A Full-length N-terminal FLAG tagged KRAS G12V in pLenti6.3/TO This paper N/A MISSION pLKO.1 shKRAS#1 Sigma TRCN0000033262 MISSION pLKO.1 shKRAS#2 Sigma TRCN0000033260 MISSION pLKO.1 luciferase shRNA control Sigma Cat# SHC007 Software and Algorithms KinTek Explorer Chemical Kinetics Software KinTek https://kintekcorp.com/software/ CCP4 software for crystallography Winn et al., 2011 http://www.ccp4.ac.uk/ QIAGEN Biomedical Genomics Workbench v4.1.1 QIAGEN https://www.qiagenbioinformatics.com/ products/biomedical-genomics-workbench/ FlowJo v10.1 Flowjo https://www.flowjo.com/solutions/flowjo MassHunter Software Agilent https://www.agilent.com/en-us/products/ software-informatics/masshunter-suite/ masshunter/masshunter-software Progenesis LC-MS software for proteomics V3.0 Waters http://www.nonlinear.com/progenesis/ Proteome Discoverer software v1.4 ThermoFisher Scientific Cat# IQLAAEGABSFAKJMAUH Skyline Targeted Mass Spec Environment v3.6 MacLean et al., 2010 https://skyline.ms GSEA (Java, v3.0) Subramanian et al., 2005 http://software.broadinstitute.org/gsea/index.jsp Project DRIVE data portal McDonald et al., 2017 https://oncologynibr.shinyapps.io/drive/ MSigDB v6.0 Subramanian et al., 2005 http://software.broadinstitute.org/gsea/index.jsp Prism v7.03 GraphPad Software N/A e3 Cell 172, 578–589.e1–e9, January 25, 2018

    Article Title: Targeting KRAS Mutant Cancers with a Covalent G12C-Specific Inhibitor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 1-(4-(6-chloro-8-fluoro-7-(2-fluorophenyl) quinazolin-4-yl)piperazin-1-yl)prop-2-en-1one (ARS-1170) This paper N/A 1-(4-(6-chloro-8-fluoro-7-(2-fluoro-6hydroxyphenyl)quinazolin-4-yl)piperazin-1-yl) prop-2-en-1-one (ARS-1323, a racemic mixture of ARS-1620/R-atropisomer) This paper N/A Labrasol (as oral vehicle) Gattefossé SAS, (Saint-Priest,France) Cat# 3074BFE, lot# 153313 Trametinib LC labs Cat# T-1823 Mant-GDP Sigma Cat# 69244 GDP Sigma Cat# G7127 GTP Sigma Cat# G8877 Guanosine 50-[b,g-imido]triphosphate trisodium salt hydrate (GppNp) Sigma Cat# G0635 Custom heavy isotopically labeled KRASG12C 1-169 his-tagged protein (Lys-13C6, 15N2 and Arg-13C6, 15N4) PROMISE Advanced Proteomics, Grenoble, France N/A Custom Isotopically labeled KRASG12C HeavyPeptide AQUA ThermoFisher Scientific N/A Iodoacetamide desthiobiotin This paper N/A Pierce High Capacity Streptavidin agarose ThermoFisher Scientific Cat# 20361 Critical Commercial Assays CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7573 Active Ras Detection Kit Cell Signaling Technology Cat# 8821 Deposited Data Crystallography data This paper PDB: 5V9U Transcriptome RNaseq data (fastq files and processed RPKM values) This paper GEO: GSE103021 Experimental Models: Cell Lines RAS-less mouse embryonic fibroblasts (MEFs): overexpressing KRAS 4B WT, KRAS 4B G12C, KRAS 4B G12D, and KRAS 4B G12V NIH (NCI RAS initiative at the FNLCR) Laboratories of Rachel Bagni, Matthew Holderfield, and Dan Soppet) N/A 293FT cell line for virus production ThermoFisher Scientific Cat# R70007 H358 (NCI-H358) cell line ATCC Cat# CRL-5807 MIA-PaCa2 cell line ATCC Cat# CRL-1420 SW837 cell line ATCC Cat# CCL-235 CALU1 cell line ATCC Cat# HTB-54 H23 (NCI-H23) cell line ATCC Cat# CRL-5800 H2122 (NCI-H2122) cell line ATCC Cat# CRL-5985 SW1463 cell line ATCC Cat# CCL-234 H2030 (NCI-H2030) cell line ATCC Cat# CRL-5914 H1373 (NCI-H1373) cell line ATCC Cat# CRL-5866 H1792 (NCI-H1792) cell line ATCC Cat# CRL-5895 SW1573 cell line ATCC Cat# CRL-2170 A549 cell line ATCC Cat# CCL-185 H441 (NCI-H441) cell line ATCC Cat# HTB-174 HCT116 cell line ATCC Cat# CCL-247 A375 cell line ATCC Cat# CRL-1619 (Continued on next page) Cell 172, 578–589.e1–e9, January 25, 2018 e2



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    ATCC ccl 235 calu1 cell line atcc
    Ccl 235 Calu1 Cell Line Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccl+235+calu1+cell+line+atcc/SW837/pm29373830-216-210-214
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